abstract：OBJECTIVE:To evaluate the feasibility, safety and diagnostic accuracy of pharmacologic stress of (99m)Technetium-MIBI single-photon emission computed tomography (SPECT) with intravenous adenosine triphosphate (ATP) in patients with suspected coronary artery disease. METHODS:The study group included 263 patients who we...

journal_title：Chinese medical journal

authors： He Q,Yao Z,Yu X,Qu W,Sun F,Ji F,Xu F,Qian Y

更新日期：2002-11-01 00:00:00

abstract：OBJECTIVE:To investigate the significance of telomerase activity in urine in the diagnosis and recurrence surveillance of urothelial carcinoma. METHODS:Telomerase activity in urine of 54 cases of urothelial carcinoma (urothelial carcinoma group) was estimated by polymerase chain reaction-enzyme-linked immunosorbent as...

journal_title：Chinese medical journal

authors： Li Z,Kong C,Wang P,Liu X,Liu T

更新日期：2002-11-01 00:00:00

abstract：OBJECTIVE:To study the pharmacodynamics of vecuronium,atracurium, mivacurium and rocuronium in patients with end-stage renal failure. METHODS:Forty-six patients with end-stage renal failure scheduled for renal transplantation and 53 patients with normal renal function were given either vecuronium, atracurium, mivacuri...

journal_title：Chinese medical journal

authors： Ma H,Zhuang X

更新日期：2002-11-01 00:00:00

abstract：OBJECTIVE:To study telomerase activity (TA) and its variation in bone marrow mononuclear cells from patients with myelodysplastic syndrome (MDS) at different stages in comparison with normal bone marrow cells and leukemic cells. METHODS:The TA was semi-quantitatively determined in mononuclear cells from 20 normal bone...

journal_title：Chinese medical journal

authors： Fu C,Chen Z

更新日期：2002-10-01 00:00:00

abstract：OBJECTIVE:To rapidly and economically obtain knowledge about adult stage Schistosoma japonicum (Chinese strain) expressed genes using expressed sequence tag (EST). METHODS:A directional cDNA library constructed from Schistosoma japonicum (Chinese strain) adult stage RNA was used to generate expressed sequence tags (ES...

journal_title：Chinese medical journal

authors： Bian G,Yu X,Wu Z

更新日期：2002-10-01 00:00:00

abstract：OBJECTIVE:To introduce the applied anatomy of pedicled buccal fat pad (BFP) graft and a series of histological changes in the healing process of BFP as an uncovered pedicled graft. METHODS:The healing processes of BFP are demonstrated via uncovered pedicled grafts on rabbits in histological examination. Uncovered bucc...

journal_title：Chinese medical journal

authors： Fan L,Chen G,Zhao S,Hu J

更新日期：2002-10-01 00:00:00

abstract：OBJECTIVE:To determine the relationships of Met416Val and XbaI polymorphism of muscle glycogen synthase (GYS1) gene and Trg64Arg variant of the beta(3)-adrenergic-receptor (beta(3)-AR) gene with type 2 diabetes mellitus (DM) and its intermediate phenotypes in the Chinese population. METHODS:Polymerase chain reaction-o...

journal_title：Chinese medical journal

authors： Wang G,Li Q,Niu T,Chen C,Xu X

更新日期：2002-09-01 00:00:00

abstract：OBJECTIVE:To investigate whether the decrease in expression of interleukin-2 (IL-2) after trauma is associated with changes in DNA binding activity of nuclear factor of activated T cells (NFAT) and activator protein-1 (AP-1). METHODS:Mice with closed impact injury with fracture in both hind limbs were adopted as the t...

journal_title：Chinese medical journal

authors： Luo Y,Liang H,Hu C,Xu X,Wang Z

更新日期：2002-09-01 00:00:00

abstract：OBJECTIVES:To study the status of cochlear mitochondrial DNA (mtDNA) and to determine the location of mtDNA deletion in aged mice. METHODS:We detected cochlear mtDNA in 2, 7 - 10 and 17 - 19 month old mice by nested polymerase chain reaction (PCR) and DNA sequencing. RESULTS:mtDNA3867bp deletions were found in the co...

journal_title：Chinese medical journal

authors： Zhang X,Han D,Ding D,Dai P,Yang W,Jiang S,Salvi RJ

更新日期：2002-09-01 00:00:00

abstract：OBJECTIVE:To discuss the etiology, diagnostic criteria and treatment of Nelson's syndrome. METHODS:Twenty-three patients with Nelson's syndrome who were treated in our department over the last 19 years were analyzed retrospectively. Removal of adenoma by the transsphenoidal approach was done in 21 patients and by tran...

journal_title：Chinese medical journal

authors： Xing B,Ren Z,Su C,Wang R,Yang Y,Hu Y

更新日期：2002-08-01 00:00:00

abstract：OBJECTIVE:To investigate the localization of Ca(2+)-ATPase (Ca(2+) pump) in the cochlear and its change after endolymphatic hydrops, and to study the relationship between compound action potential (CAP) threshold and the Ca(2+)-ATPase activety. METHODS:The left endolymphatic sac was ablated to induce endolymphatic hyd...

journal_title：Chinese medical journal

authors： Zhang S,Yang W,Sun J,Zhao C,Zhou N

更新日期：2002-08-01 00:00:00

abstract：OBJECTIVES:To formulate an equation for fine mapping of disease loci under complex conditions and determine the marker-disease distance in a specific case using this equation. METHODS:Lewontin's linkage disequilibrium (LD) measure D' was used to formulate an equation for mapping disease genes in the presence of phenoc...

journal_title：Chinese medical journal

authors： Gong G,Haynatzki G,Deng HW,Recker RR,Mordeson J,Cheng SC,Fong N

更新日期：2002-08-01 00:00:00

abstract：OBJECTIVE:To study the prevalence of skin positivity to purified protein derivative (PPD) in human immunodeficiency virus (HIV)-infected patients in Hong Kong. METHODS:Consecutive clients of an HIV clinic were administered the PPD test and 2 units of PPD-RT23 were used. The area of induration was then measured in 48 t...

journal_title：Chinese medical journal

authors： Chan KC,Tang HW,Wong K

更新日期：2002-07-01 00:00:00

abstract：OBJECTIVE:To investigate the possibility of using melanoma antigen-1 (MAGE-1) peptide as a tumor vaccine to treat hepatocellular carcinoma (HCC). METHODS:The expressions of MAGE-1 in 8 HCC cell lines and in liver cancer tissue from a patient were detected using RT-PCR. The type of human leucocyte antigen I(HLA I) of b...

journal_title：Chinese medical journal

authors： Lu J,Leng X,Peng J,Mou D,Pang X,Shang X,Chen W

更新日期：2002-07-01 00:00:00

abstract：OBJECTIVE:To assess the feasibility of detecting SYT-SSX fusion transcripts in paraffin-embedded tissues of synovial sarcoma by reverse transcription-polymerase chain reaction (RT-PCR). METHODS:RT-PCR was used to amplify the SYT-SSX fusion transcripts using archival formalin-fixed paraffin-embedded tumor specimens fro...

journal_title：Chinese medical journal

authors： Wei Y,Wang J,Zhu X,Shi D,Hisaoka M,Hashimoto H

更新日期：2002-07-01 00:00:00

abstract：OBJECTIVE:To determine the in vitro expression of interleukin-12 (IL-12) and its effect on signal transducers and activators of transcription (STAT) signaling molecules in peripheral blood mononuclear cells (PBMCs) in patients with systemic lupus erythematosus (SLE). METHODS:Peripheral blood mononuclear cells in 39 pa...

journal_title：Chinese medical journal

authors： Li Z,Li Y,Huang L,Xu H,Yu X,Ye R

更新日期：2002-06-01 00:00:00

abstract：OBJECTIVE:To study the surgical technique and results of laryngeal function preservation in treatment of hypopharyngeal carcinoma. METHODS:A retrospective review of 305 patients with malignant neoplasms of the hypopharynx (279 males, 26 females, age ranging from 14 to 77 years) was performed from 1978 to 1996. In the ...

journal_title：Chinese medical journal

authors： Wang T,Li X,Lu Y,Yu Z

更新日期：2002-06-01 00:00:00

abstract：:Cerebral cysticercosis is the most common worldwide parasitic infection of the central nervous system. Intraventricular involvement is apparent in 15% to 28.8% of cases with neural compartment infestation.' Although different forms of the disease (parenchymatous, subarachnoid, and mixed form ) have been treated succes...

journal_title：Chinese medical journal

authors： Zhu L,Weng X,Shi Y,Pan X,Mo L

更新日期：2002-06-01 00:00:00

abstract：OBJECTIVE:To observe the characteristics of coronary microvascular lesions (CML) in the autopsied elderly cases with hypertensive left ventricular hypertrophy (LVH) and the difference of CML among the groups of essential hypertension (EHT), coronary heart disease (CHD) and diabetes (NIDDM) also with LVH. METHODS:A ret...

journal_title：Chinese medical journal

authors： Li X,Li R,Yu W,Shi H,Wei L

更新日期：2002-05-01 00:00:00

abstract：OBJECTIVE:To clarify the role of these cyclins in human gastric cancer. METHODS:38 gastric cancer patients, 29 first degree relatives of gastric cancer patients, as well as 18 healthy subjects were included. The mRNA expression of cyclins D1, D2, D3 and E in gastric biopsies was evaluated by RT-PCR analysis using spec...

journal_title：Chinese medical journal

authors： Yu J,Miehlke S,Ebert MP,Szokodi D,Wehvnignh B,Malfertheiner P,Ehninger G,Bayerdoerffer E

更新日期：2002-05-01 00:00:00

abstract：OBJECTIVE:To investigate the role of a potential diabetes-related mitochondrial region, which includes two previously reported mutations, 3243A-->G and 3316G-->A, in Chinese patients with adult-onset type 2 diabetes. METHODS:A total of 277 patients and 241 normal subjects were recruited for the study. Mitochondrial nt...

journal_title：Chinese medical journal

authors： Yang T,Lam CW,Tsang MW,Tong SF,Kam GY,Chan LY,Poon PM,Wu X,Pang CP

更新日期：2002-05-01 00:00:00

abstract：OBJECTIVE:To determine the feasibility and accuracy of high-frequency ultrasound in evaluating the left ventricular (LV) structure and function in normal and pressure overload rats and to examine the changes of the left ventricle during its transition from hypertrophy to heart failure. METHODS:Thirty-eight female rats...

journal_title：Chinese medical journal

authors： Xu F,Wang J,Bai X,Yang J,Bai S

更新日期：2002-04-01 00:00:00

abstract：OBJECTIVE:To detect occult metastases in lymph nodes from patients with colorectal carcinoma and its clinical significance. METHODS:The metastases in 260 lymph nodes from 39 histologically verified colorectal cancer patients were studied by both hematoxylin-eosin (HE) staining and cytokeratin-20 (CK20) specific RT-PCR...

journal_title：Chinese medical journal

authors： Liu Z,Ye X,Bi W,Wang M,Li Y,Chen T

更新日期：2002-04-01 00:00:00

abstract：OBJECTIVE:To investigate the effect of active immunotherapy with anti-idiotypic vaccine in patients with nasopharyngeal carcinoma (NPC). METHODS:Anti-idiotypic antibodies (2H4/5D3) bearing the internal image of the NPC antigen were used in active immunotherapy in NPC patients receiving radiotherapy. Antibodies and cyt...

journal_title：Chinese medical journal

authors： Li G,Xie L,Zhou G,Fu H,Zhou J,Sun Q

更新日期：2002-04-01 00:00:00

abstract：OBJECTIVE:To investigate the feasibility and features of 3 catheter-mediated approaches of gene transfer into heart, including direct myocardial injection (DMI), coronary artery perfusion (CAP), and intrapericardial cavity injection (ICI). METHODS:Fifteen dogs were used, and 0.3 ml (1 x 10(9) pfu) of an adenovirus (Ad...

journal_title：Chinese medical journal

authors： Li J,Li G,Huang C,Jiang H,Tang Q,Xu J,Xia H,Wang J

更新日期：2002-04-01 00:00:00

abstract：OBJECTIVE:To explore the management of cerebral ischemia caused by Takayasu's arteritis. METHODS:Ninety-three cases treated from June 1984 to September 1999 at the General Post & Telecom Hospital, the Sir Run Run Shaw Hospital, the First Affiliated Hospital of Zhejiang University, the Second Medical College of Beijing...

journal_title：Chinese medical journal

authors： Wang Z,Shen L,Yu J,Gu Y,Wang S,Guan H,Wu Q,Zhang X,Li M,Wu J,Li G,Pan S,Zhang H,Jin W

更新日期：2002-03-01 00:00:00

abstract：OBJECTIVE:To explore the characteristics of Helicobacter pylori (H. pylori) antigen in serum and to evaluate its clinical diagnostic value. METHODS:Enzyme-linked immunosorbant assay (ELISA) was developed to detect the soluble H. pylori antigen (S-Hp) and circulatory specific H. pylori antigen immunocomplexes (Hp-IC) i...

journal_title：Chinese medical journal

authors： Zhu Y,Lin J,Li D,Du Q,Qian K,Wu Q,Zheng S

更新日期：2002-03-01 00:00:00

abstract：OBJECTIVE:To investigate the compositions of Th1/Th2/Th3 cells in chronic hepatitis B virus (HBV)-infected individuals by determining the expression of interleukin-4 (IL-4), inetrferon-gamma (IFN-gamma), and transform growth factor-beta (TGF-beta) in single CD4(+) T cells isolated from peripheral blood mononuclear cell...

journal_title：Chinese medical journal

authors： Jiang R,Feng X,Guo Y,Lu Q,Hou J,Luo K,Fu N

更新日期：2002-03-01 00:00:00

abstract：OBJECTIVE:To investigate the effect of L-arginine (L-arg) on early compensatory renal growth (CRG), tubulointerstitial accumulation of extracellular matrix (ECM), long term survival rate and renal scarring in rats with 5/6 nephrectomy (SNx). METHODS:The experiment included four groups of rats (n = 5 each group): (1) S...

journal_title：Chinese medical journal

authors： Liu B,Haylor J,El Nahas AM

更新日期：2002-02-01 00:00:00

abstract：OBJECTIVE:To investigate the mechanism of multidrug resistance (MDR) in a human glioma cell and methods for overcoming multi-drug resistance. METHODS:MDR cell line C(6)/adr was established. The expression of the mdr-1 gene and its P-glycoprotein (P-gp) in the C(6)/adr cell line was observed by RT-PCR and flow cytometr...

journal_title：Chinese medical journal

authors： Liu F,Wang C,Zhang Q,Li J,Zhang Y

更新日期：2002-02-01 00:00:00

abstract：OBJECTIVE:To investigate the expression of Toll-like receptor 2 (TLR2) and Toll-like receptor 4 (TLR4) and the effect of lipopolysaccharide (LPS) on their expression in cultured endothelial cells. METHODS:Total RNA was extracted from ECV304 cells and isolated human umbilical vein endothelial cells (HUVECs) exposed to ...

journal_title：Chinese medical journal

authors： Yang Q,Zhu P,Wang Z,Jiang J

更新日期：2002-02-01 00:00:00

abstract：OBJECTIVE:To test the immunosuppressive effect of cyclosporine (Cs) in a polymer placed in the anterior chamber of corneal allograft recipients. METHODS:Wistar inbred rats with vascularized corneas were recipients of corneal allografts from Sprague-Dawley donor rats. Rats underwent penetrating keratoplasty and were di...

journal_title：Chinese medical journal

authors： Xie L,Shi W,Wang Z,Bei J,Wang S

更新日期：2002-01-01 00:00:00

abstract：OBJECTIVE:To define the prevalence, severity and risk factors for lead poisoning in new immigrant children from the mainland of China to Hong Kong, China. METHODS:New immigrant children from the mainland of China under 18 years of age were invited to join the study. Their growth parameters and venous blood lead levels...

journal_title：Chinese medical journal

authors： Ling S,Chow C,Chan A,Tse K,Mok K,Ng S

更新日期：2002-01-01 00:00:00

abstract：OBJECTIVE:To present a special case with the karyotype and molecular marker of acute myeloid leukemia (AML)-M2 who was induced to complete remission by all-trans retinoic acid (ATRA) alone. METHODS:A recently hospitalized young female patient with acute leukemia was initially diagnosed as M3 subtype based on morpholog...

journal_title：Chinese medical journal

authors： Chen Z,Wang Y,Wang W,Gong J,Xue Y

更新日期：2002-01-01 00:00:00

abstract：OBJECTIVE:To evaluate the efficacy and the mechanism of application of selective head cooling on neuronal morphological damage during postischemic reperfusion in a rabbit model. METHODS:168 New Zealand rabbits were randomized into three groups. Group I [n = 24, (38 +/- 0.5) degrees C, non-ischemic control]; Group II [...

journal_title：Chinese medical journal

authors： Duan M,Li D,Xu J

更新日期：2002-01-01 00:00:00

abstract：OBJECTIVE:To observe the long-term follow-up of 72 patients with transient ischemic attack (TIA) and evaluate the clinical significance of neurovascular surgical indication. METHODS:Seventy-two patients with TIA collected from years 1959 to 1977 were followed up by means of face-to-face communication with the patients...

journal_title：Chinese medical journal

authors： Gong T,Wang X

更新日期：2001-12-01 00:00:00

abstract：OBJECTIVE:To investigate the prevalence and features of active human cytomegalovirus (HCMV) infection in children with systemic lupus erythematosus (SLE) and evaluate the diagnostic value of the HCMV using antigenemia assay, serum polymerase chain reaction (PCR) and serology test. METHODS:Twenty-one SLE children under...

journal_title：Chinese medical journal

authors： Zhang C,Shen K,Jiang Z,He X

更新日期：2001-12-01 00:00:00

abstract：OBJECTIVE:To investigate the efficacy of continuous renal replacement therapy (CRRT) versus intermittent hemodialysis (IHD) in patients with severe acute renal failure (ARF). METHODS:One hundred and ninety-three severe ARF patients who received renal support between December 1978 and December 1998 were involved in thi...

journal_title：Chinese medical journal

authors： Ji D,Gong D,Xie H,Xu B,Liu Y,Li L

更新日期：2001-11-01 00:00:00

abstract：OBJECTIVE:To detect the virus in the feces and sera of patients in an outbreak of enterically transmitted non-A, non-E hepatitis, and this review covers the epidemiologic features and experimental infection of this novel virus. DATA SOURCES:Data sources come from our own work on this subject, published and unpublished...

journal_title：Chinese medical journal

authors： Luo K,Zhang L

更新日期：2001-11-01 00:00:00

abstract：OBJECTIVE:To identify new recombinant antigens with potential for diagnosis of hemorrhagic fever with renal syndrome (HFRS) and establish reverse transcription-polymerase chain reaction-restriction fragments length polymorphism (RT-PCR-RFLP) for genotyping of hantavirus. METHODS:One group of primers was used to clone ...

journal_title：Chinese medical journal

authors： Tang J,Cao M,Tang T,Wang C,Wei C,Lei W

更新日期：2001-10-01 00:00:00